Determination of ammonium in natural waters by phenol. Determination of ammonia in natural waters by the phenolhypochlorite method 1 1 this research was fully supported by u. The concentration of hypo chlorite and the measurement of ph are described in the text. Aha showed a weak anti proteus mirabilis activity the mic 614. The method employs the socalled indophenolblue reaction between ammonia, phenol and. Also, their inhibitory effect on urease was determined by measuring ammonia production as an indicator of urease activity using the indophenol method as described by weatherburn. An improved method for the determination of ammonia in seawater article pdf available in marine chemistry 203. Curves f to a to color development in the estimation of ammonia by the phenol hypochlorite reaction. Pdf an improved method for the determination of ammonia. Reaction rate method for ammonia and blood urea nitrogen utilizing a pentacyanonitrosylferrate catalyzed berthelot reaction. Catalytic conversion of nitrogen to ammonia by an iron. Alkaline phenol and hypochlorite react with ammonia. A new phenolhypochlorite reaction for ammonia nature.
Unfortunately this reaction cannot be applied directly to biological fluids, because certain aminoacids, alkylamines and ammonium salts can react to a certain extent. A salicylatehypochlorite method for determining ammonia. Application of the phenolhypochlorite reaction to measurement of ammonia concentrations in kjeldahl digests of serum and various tissues. The phenol hypochlorite reaction for ammonia was first described by d. Catalytic conversion of nitrogen to ammonia by an iron model complex. A salicylatehypochlorite method for determining ammonia in. Pdf selectivity of reaction among chlorine, ammonia, and.
A study was made of the effects of changes in phenol, hypochlorite and catalyst concentrations on the indophenol blue reaction to determine ammonianitrogen. Total ammonianitrogen nh 4 n in artificial seawater was determined spectrophotometrically by a method in which indophenol blue is produced by the reaction of ammonia with salicylate and hypochlorite, in the presence of sodium nitroprusside. The blue color formed is intensified with sodium nitroprusside and measured colorimetrically. Some aspects of the phenolhypochlorite reaction as applied to. The method we will use is called the indophenol blue method or phenate method. The blue color of indophenol obtained by the reaction at high ph of ammonia, phenol, and hypochlorite has been studied. Weatherburn mw 1967 phenolhypochlorite reaction for determination of ammonia. Alkaline phenol and hypochlorite react with ammonia to form indophenol blue that is proportional to the ammonia concentration. All samples contained 10 y of ammonia nitrogen and the volume of solution during color development was 10 ml.
Al though his procedure is reproducible and sensitive, it has the disadvantage of requir ing distillation. Further study of the reaction has led to a several fold increase in its. Phenolhypochlorite reaction for determination of ammonia. Formation of ammonia in the reactions of a tungsten dinitrogen with ruthenium dihydrogen complexes under mild reaction conditions1. This method covers the determination of ammonia in drinking, ground. Spectrophotometrlc data show that within the ph range 9.
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